人力資源與數位學習科技研究所

Abstract

　　 Sister chromatid exchanges (SCE) involve breakage of both DNA strands, followed by an exchange of whole DNA duplexes. The formation of SCE correlates closely with recombinational repair and the induction of point mutations, gene amplification and cytotoxicity. Cytogenetic analyses are almost always based on examination of chromosomes fixed during mitotic metaphase. A common task in Cytogenetic tests is the classification of human chromosomes by specific features. There are two arms in a chromosome. The shorter one is named P arm and the longer one is named Q arm. Sister chromatids are attached at an area called the centromere. Level Set method is a numerical technique that can follow the evolution of contours and interfaces. Analyzing regularities and singularities of shapes is an indirect skeletonization method that can reduce unwanted skeletonization artifacts. We use these two methods to extract of boundaries and skeletons of SCE images that can be further used for classification and analyses of human chromosomes.